Question 1

Who invented the first crude microscope by grinding glass?

Accepted Answer

A)  Redi
B)  Lister
C)  Schultz and Schwann
D)  Leeuwenhoek
E)  PasteurF) A) and B)
G) A) and C)

Question 2

At the end of the Gram stain,gram-positive bacteria will be seen as pink cells.

Accepted Answer

A) True 
 B)False

Question 3

Which of the following magnifies the specimen to produce the virtual image of the specimen?

Accepted Answer

A)  objective lens
B)  ocular lens
C)  condenser
D)  body
E)  iris diaphragmF) None of the above
G) A) and E)

Question 4

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is

Accepted Answer

A)  isolation.
B)  inoculation.
C)  immunization.
D)  infection.
E)  contamination.F) D) and E)
G) B) and D)

Question 5

Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens.

Accepted Answer

A) True 
 B)False

Question 6

Newly inoculated cultures must be _____ at a specific temperature and time to encourage growth.

Accepted Answer

A)  streaked
B)  poured
C)  incubated
D)  spreadE) All of the above
F) A) and B)

Question 7

The procedures for culturing a microorganism require the use of a microscope.

Accepted Answer

A) True 
 B)False

Question 8

Basic dyes are

Accepted Answer

A)  attracted to the acidic substances of bacterial cells.
B)  anionic.
C)  used in negative staining.
D)  repelled by cells.
E)  dyes such as India ink and nigrosin.F) A) and B)
G) None of the above

Question 9

Robert Koch and his lab workers first used agar in bacteriological media.Why did they find this media so much better than previous materials used to grow bacteria?

Accepted Answer

A)  Bacteria,growing on the solidified media,did not degrade or break down the medium.
B)  Agar could convert from liquid to solid form,and back again,very easily.
C)  The food material that Koch was previously using in the lab was much more expensive than agar.
D)  Agar is a great source of nutrients for bacteria.E) B) and C)
F) B) and D)

Question 10

Which type of microscope shows cells against a bright background and also shows intracellular structures of unstained cells based on their varying densities?

Accepted Answer

A)  bright-field
B)  dark-field
C)  phase-contrast
D)  differential interference
E)  electronF) B) and C)
G) A) and E)

Question 11

A reducing medium contains

Accepted Answer

A)  sugars that can be fermented.
B)  extra oxygen.
C)  hemoglobin,vitamins,or other growth factors.
D)  substances that remove oxygen.
E)  inhibiting agents.F) D) and E)
G) All of the above

Question 12

A nutrient medium that contains at least one ingredient that is not chemically definable is termed

Accepted Answer

A)  complex.
B)  reducing.
C)  enriched.
D)  synthetic.E) None of the above
F) A) and D)

Question 13

_____ has the same optical qualities as glass and thus prevents refractive loss of light as it passes from the slide to the objective lens.

Accepted Answer

A)  The scanning objective
B)  The oil objective
C)  The slide
D)  Immersion oil
E)  The ocularF) A) and D)
G) None of the above

Question 14

Which type of microscope does not use light in forming the specimen image?

Accepted Answer

A)  bright-field
B)  dark-field
C)  phase-contrast
D)  fluorescence
E)  electronF) A) and D)
G) A) and E)

Question 15

The type of microscope in which you would see brightly illuminated specimens against a black background is

Accepted Answer

A)  bright-field.
B)  dark-field.
C)  phase-contrast.
D)  fluorescence.
E)  electron.F) C) and E)
G) C) and D)

Question 16

A microbiologist inoculates Staphylococcus aureus into a culture medium.Following incubation,both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.What is the most likely explanation?

Accepted Answer

A)  The microbiologist used too much inoculum.
B)  The culture is contaminated.
C)  The incubation temperature was incorrect.
D)  The culture medium must be selective.
E)  The culture medium must be differential.F) A) and B)
G) B) and C)

Question 17

A common medium used for growing fastidious bacteria is

Accepted Answer

A)  blood agar.
B)  trypticase soy agar.
C)  mannitol salt agar.
D)  MacConkey medium.
E)  reducing medium.F) A) and B)
G) A) and C)

Question 18

The specimen preparation that is best for viewing cell motility is

Accepted Answer

A)  hanging drop.
B)  fixed,stained smear.
C)  Gram stain.
D)  negative stain.
E)  flagellar stain.F) A) and B)
G) A) and C)

Question 19

A microbiologist decides to use a nutrient medium that contains thioglycollic acid.What type of microbe is she attempting to culture?

Accepted Answer

A)  fastidious
B)  gram-positive
C)  anaerobic
D)  gram-negative
E)  aerobicF) A) and B)
G) B) and C)

Question 20

Agar is a complex polysaccharide that comes from a/an

Accepted Answer

A)  green plant.
B)  fungus.
C)  mold.
D)  algae.
E)  Euglena.F) A) and D)
G) A) and C)

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

A microbiologist decides to use a nutrient medium that contains thioglycollic acid.What type of microbe is she attempting to culture?

Correct AnswerverifiedShow Answer

Which type of microscope does not use light in forming the specimen image?

Correct AnswerverifiedShow Answer

_____ has the same optical qualities as glass and thus prevents refractive loss of light as it passes from the slide to the objective lens.

Correct AnswerverifiedShow Answer

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is

Correct AnswerverifiedShow Answer

Basic dyes are

Correct AnswerverifiedShow Answer

A nutrient medium that contains at least one ingredient that is not chemically definable is termed

Correct AnswerverifiedShow Answer

Newly inoculated cultures must be _____ at a specific temperature and time to encourage growth.

Correct AnswerverifiedShow Answer

A common medium used for growing fastidious bacteria is

Correct AnswerverifiedShow Answer

A microbiologist inoculates Staphylococcus aureus into a culture medium.Following incubation,both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.What is the most likely explanation?

Correct AnswerverifiedShow Answer

Which type of microscope shows cells against a bright background and also shows intracellular structures of unstained cells based on their varying densities?

Correct AnswerverifiedShow Answer

Who invented the first crude microscope by grinding glass?

Correct AnswerverifiedShow Answer

At the end of the Gram stain,gram-positive bacteria will be seen as pink cells.

Correct AnswerverifiedShow Answer

Robert Koch and his lab workers first used agar in bacteriological media.Why did they find this media so much better than previous materials used to grow bacteria?

Correct AnswerverifiedShow Answer

Agar is a complex polysaccharide that comes from a/an

Correct AnswerverifiedShow Answer

Which of the following magnifies the specimen to produce the virtual image of the specimen?

Correct AnswerverifiedShow Answer

Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens.

Correct AnswerverifiedShow Answer

The procedures for culturing a microorganism require the use of a microscope.

Correct AnswerverifiedShow Answer

The type of microscope in which you would see brightly illuminated specimens against a black background is

Correct AnswerverifiedShow Answer

The specimen preparation that is best for viewing cell motility is

Correct AnswerverifiedShow Answer

A reducing medium contains

Correct AnswerverifiedShow Answer

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