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The specimen preparation technique that is best for viewing cell motility is the ______.


A) flagellar stain
B) Gram stain
C) negative stain
D) hanging drop
E) fixed, stained smear

F) A) and B)
G) A) and C)

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Differential media result in which of the following observable characteristics?


A) Differently colored colonies
B) Different media color after incubation
C) Chemical precipitates
D) Gas bubbles
E) All of the choices are correct.

F) C) and D)
G) All of the above

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Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.


A) ocular; oil immersion
B) scanning; objective
C) ocular; objective
D) objective; ocular

E) B) and C)
F) All of the above

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A scanning confocal microscope


A) produces specimen images on electron micrographs.
B) uses dyes that emit visible light when excited by ultraviolet radiation.
C) uses ultraviolet light to form a specimen image.
D) scans narrow planes and can build a three-dimensional image of the specimen.

E) A) and B)
F) None of the above

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Table 3.6 in your textbook uses Paramecium to illustrate several types of light microscopy.Each image is magnified 230X and measures about 4 cm.The actual size of a Paramecium is about ______.


A) 920 μ\mu m
B) 0.2 μ\mu m
C) 175 μ\mu m
D) 57.5 μ\mu m
E) 4,000 μ\mu m

F) D) and E)
G) B) and E)

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The Five I's of studying microorganisms include all of the following except ______.


A) identification
B) isolation
C) incubation
D) infection
E) inoculation

F) B) and C)
G) A) and E)

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All of the following pertain to the fluorescence microscope except


A) it is a type of compound microscope.
B) it is used to diagnose certain infections.
C) it uses electrons to produce a specimen image.
D) it requires the use of dyes like acridine or fluorescein.
E) it requires an ultraviolet radiation source.

F) C) and E)
G) None of the above

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A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium. Following incubation, only the E. coli grows in the culture. What is the most likely explanation?


A) The culture medium was selective.
B) The culture medium was differential.
C) The microbiologist used too much inoculum.
D) The culture was contaminated.
E) The incubation temperature was incorrect.

F) All of the above
G) A) and E)

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All of the following are examples of different types of microbiological media except ______.


A) agar
B) enriched
C) broth
D) Petri dish
E) semisolid

F) A) and E)
G) None of the above

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Agar is a complex polysaccharide that comes from a/an ______.


A) bacterium
B) algae
C) fungus
D) marine invertebrate
E) green plant

F) A) and E)
G) None of the above

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Comparing transmission electron microscopy with scanning electron microscopy, the following statement is true.


A) Transmission EM is used for internal detail of cells and subcellular structures.
B) Transmission EM gives detail of the external architecture of cells.
C) Transmission EM requires dyes.
D) Transmission EM can provide good images of bacteria but not viruses.

E) None of the above
F) A) and D)

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Bacterial cultures are easily identified from their microscopic appearance.

A) True
B) False

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Who invented the first crude microscope by grinding glass?


A) Lister
B) Schultz and Schwann
C) Leeuwenhoek
D) Redi
E) Pasteur

F) A) and B)
G) C) and D)

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Fixed smears of specimens are required to perform the Gram stain or the endospore stain.

A) True
B) False

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